FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12520619
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCC652640.5212521840972878TruSeq Adapter, Index 7 (97% over 37bp)
CTTGTGGAAAGGACGAAACACCGGTCCGCAGGTATGCTTGCGTGAATTCCA222300.17754713245407436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG38200.043.58408722
ACCGGTC39550.042.6083720
GTCCGCA39400.041.85692624
GGTCCGC40050.041.6270223
GGACGAA41100.041.5492411
CACCGGT41400.040.8130719
GCTTGCG41000.040.49786436
CCGGTCC41150.040.4595921
CTTGCGT41400.040.10658337
GACGAAA42350.040.0037912
AGGACGA42750.039.9982110
ACGAAAC43000.039.2421213
AAGGACG43700.039.231819
CGAAACA43400.039.1396414
CGCAGGT41300.039.00629827
CGTATGC100200.038.86802344
TTGCGTG42750.038.6298538
ACACCGG43650.038.6062118
CGTGAAT42750.038.47196241
CTCGTAT100500.038.34903342
Produced by FastQC (version 0.11.5)