FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14066639
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCC769100.5467546298728502TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG20800.042.61784722
ACCGGTC22100.040.6199420
GGTCCGC22100.040.21272323
GTCCGCA22200.039.72754324
CTCGTAT97900.039.59732442
TCTCGTA95600.039.4909341
CGTATGC99500.039.48066344
CACCGGT23350.038.73448619
TCGTATG101650.038.51280643
CCGGTCC23150.038.48600421
ACACCGG24100.038.0891918
ATCTCGT99100.038.05078540
GCTTGCG24050.037.23288336
CGCAGGT24200.037.19068527
GGACGAA24850.037.12082711
AATCTCG97050.036.3739739
TTGCGTG24950.036.1607238
GTATGCC109100.036.15049745
ACGATCG7700.035.9421431
CGTCTGA112950.035.5357716
Produced by FastQC (version 0.11.5)