FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13596623
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14200.026.4596144
TCTCGTA13400.026.19200541
CTCGTAT14100.026.16828342
TCGTATG15800.023.63741343
ATCTCGT17250.020.21590240
GTATGCC22200.016.82382845
CATCTCG21400.016.61092839
TATGCCG4200.016.60665345
CTTAGGC26300.014.37172333
GCATCTC30750.012.07259338
TTAGGCA32400.011.73550634
ACTTAGG33200.011.72370732
CGTCTGA32200.011.59779916
GGCATCT31450.011.58927137
CCAGTCA35550.010.82174926
CAGTCAC37200.010.52303227
AGGCATC35250.010.40375736
CACTTAG36150.010.20645431
GTCACTT38950.09.87706729
AGTCACT39800.09.77907128
Produced by FastQC (version 0.11.5)