FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8821486
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.021.57513845
CTCGTAT6400.017.928442
TCTCGTA6850.015.7652941
CGTATGC7350.015.30528444
TCGTATG7750.014.80522143
TTCGATC8250.013.63569436
CGTTTCG8000.013.49933633
ATCTCGT9550.011.54380440
GTCACGT9750.011.0761929
AGTCACG10250.010.75508128
TTTCGAT11750.010.72306135
GTATGCC10950.010.68483145
ACGTTTC11700.010.19180732
GTTTCGA11450.09.43201234
CGTCTGA37550.09.34744845
CACGTTT13000.08.82628931
TCACGTT12300.08.77985830
ACACGTC72450.08.72662645
CACGTCT63450.08.33325545
CACACGT96800.08.27471545
Produced by FastQC (version 0.11.5)