FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8280321
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT12100.025.28761742
TCTCGTA11150.025.22273341
CGTACGA10650.024.29419934
CCGTACG10800.023.95678133
CGTATGC13500.023.33182144
TCGTATG14400.022.49854343
TATGCCG4050.022.22078145
GTACGAT11050.022.19326635
TACGATC11200.021.49427236
ACCGTAC12200.021.2076432
GTATGCC16400.019.34325845
ATCTCGT15000.018.74889840
GTCACCG15300.017.79296529
CACCGTA15600.016.87390531
ACGATCT22550.011.87291737
CGTCTGA28000.011.73138316
CAGTCAC30650.011.37772927
AGTCACC27200.011.24927128
CCAGTCA32200.010.89991526
TCACCGT26700.010.4487630
Produced by FastQC (version 0.11.5)