FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8315771
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCC93550.11249708535744911TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18900.027.97439444
CTCGTAT19400.027.36937342
TCGTATG20000.026.435843
TCTCGTA18800.025.7297341
ATCTCGT21050.023.19328340
GTATGCC24450.021.9004345
TATCGGA1752.5993359E-916.760981
TATGCCG5200.014.71059245
CGTCTGA38150.014.38963816
GCTCGTA1605.038575E-614.06159741
CCAGTCA40550.013.87087226
AGTCACC34950.013.71156528
CAGTCAC40950.013.68043927
AGATCTC33650.012.7704338
ACGTCTG43900.012.6073915
TCTGAAC46050.012.06762818
GTCACCA38500.011.74599829
CCGATGT1350.00401242211.66598733
AAGATCT36600.011.618175537
CACGTCT48250.011.42413714
Produced by FastQC (version 0.11.5)