FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12571571
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16050.022.98916645
CTCGTAT24100.018.29757144
TCTCGTA24450.017.39151443
TATCTCG25300.016.98506741
TCGTATG26650.016.46234945
ATCTCGT27800.015.53856242
CGTCTGA41900.013.79982216
ACGTCTG50050.011.7774615
CCAGTCA50400.011.60639226
CAGTCAC51900.011.44434627
CACGTCT56550.010.46351314
TCTGAAC58200.010.39881218
TATCGGA2201.2748044E-410.2561851
ACACGTC56750.010.18876813
GTCACAT49550.09.489829
GTCTGAA65700.09.38295517
CTGAACT65750.09.20472719
CTCCAGT66250.09.16921824
GTATGCC15350.08.7942245
TCCAGTC73000.08.44465925
Produced by FastQC (version 0.11.5)