FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10950594
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC1158881.058280491450966TruSeq Adapter, Index 9 (97% over 36bp)
CTTGTGGAAAGGACGAAACACCGGTCCGCAGGTATGCTTGCGTGAATTCCA192850.1761091681419291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG36150.043.25506222
ACCGGTC36450.043.08423220
GTCCGCA36950.042.0749924
CACCGGT37600.041.94600319
GGTCCGC37700.041.6557123
GGACGAA38750.041.51401511
CTTGCGT38500.040.78976437
CGTATGC155650.040.4301944
CCGGTCC38750.040.41084721
CTCGTAT154700.040.38759642
TCTCGTA149850.040.3434841
TATCTCG138000.040.07423439
ACGAAAC40000.040.04759613
GCTTGCG39350.040.0230136
AGGACGA40350.039.9236210
AAGGACG40250.039.8551189
TCGTATG158000.039.81461343
ATCTCGT152200.039.73534840
GACGAAA40500.039.66428812
CGTGAAT39650.039.55050341
Produced by FastQC (version 0.11.5)