FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12544362
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT12300.025.60787642
TCTCGTA13050.023.96366141
CGTATGC13450.023.92065244
TCGTATG14900.021.89427843
ATCTCGT17550.018.07573940
TCACGAT17100.017.76234835
GTATGCC18900.017.3802345
CATCACG22950.014.60700333
TATGCCG5050.012.02920345
GTCACAT29100.011.05571329
CCAGTCA34900.09.92763826
CAGTCAC34150.09.75011127
ACGATCT43350.08.92700137
CGTCTGA44850.08.879230545
CACGATC43650.08.86564636
ACACGTC81950.08.81293445
ACGTCTG59650.07.95859645
TCACATC42350.07.7029130
AGCACAC168850.07.555208745
TCTGAAC45000.07.548934518
Produced by FastQC (version 0.11.5)