FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11278571
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGGAAAGGACGAAACACCGGTCCGCAGGTATGCTTGCGTGAATTCCA222160.1969753083081181No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCC121330.1075756848983794TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG37950.043.57508522
ACCGGTC39300.042.47897720
GGTCCGC39500.041.86517323
GGACGAA40950.041.4816211
GTCCGCA39900.041.38908424
CACCGGT40400.041.37805619
CCGGTCC40300.041.1457621
CGCAGGT39950.040.71977627
AGGACGA41950.040.43915610
GCTTGCG40850.040.31621636
TTGCGTG41050.040.17477438
CTTGCGT40900.040.1569137
ACGAAAC42250.039.88557413
GACGAAA42650.039.7752612
ACACCGG42250.039.72599818
TGCGTGA41350.039.66565739
TCCGCAG41450.039.62617525
AAGGACG42800.039.5834739
CCGCAGG42000.039.3751226
CGTGAAT41900.039.35977641
Produced by FastQC (version 0.11.5)