FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11272404
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGCC1164621.0331602735317151TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGCCG171440.15208823246576328TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT162650.036.5039442
CGTATGC164300.036.452344
TCTCGTA156050.036.37557241
TCGTATG165500.036.16080543
ACCGGAT154600.035.90178335
CGGATCT154250.035.73528737
CCGGATC154600.035.7271536
ATCTCGT159200.035.599340
CACCGGA166250.034.9691734
GTATGCC171750.034.87110545
CCACCGG170800.034.19568333
ACCACCG172750.033.7315432
TATGCCG29200.033.67076545
GGATCTC170500.032.5141738
CGTCTGA190400.031.78628516
AGTCACC189200.031.19116628
ACGTCTG197200.030.66738715
CCAGTCA196450.030.64703826
CAGTCAC197350.030.50727527
GTCACCA193100.030.21166829
Produced by FastQC (version 0.11.5)