FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005099984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005099984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6885161
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGCA5316167.721184733370795No Hit
TGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCG3726905.412945318199531No Hit
CTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC3335704.844766883446879No Hit
GAGTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG3151664.577467396913449No Hit
GTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC3143374.5654270103487775No Hit
ATCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCC3010044.3717786700993635No Hit
ATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC2972834.317734908450217No Hit
TCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCG2921984.243880426325543No Hit
GTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2827034.105975154393629No Hit
TCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACA2724243.9566830753848743No Hit
ATCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCAC2623903.8109493735876327No Hit
CTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2594683.7685102788446048No Hit
CACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG2540763.690196932214076No Hit
GAGTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT2345583.406717722359724No Hit
ATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2275093.304338126588471No Hit
CACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT2040652.9638377374181952No Hit
ACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1946542.82715248053023No Hit
GACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGG1929532.802447175890295No Hit
GATCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACC1859672.7009825914020023No Hit
GCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGCAG1849592.686342410874633No Hit
AGTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1744742.534058390210483No Hit
ACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1558582.263679818089947No Hit
GATCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCA1419942.062319239884151No Hit
GATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1293811.8791281714399997No Hit
AGTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1264461.8365002648449325No Hit
AGATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG1261731.8325352159521036No Hit
GATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1162761.688791300595585No Hit
AGATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT872021.2665208555035967No Hit
GAGTGCGACGTCGATGACCTGGACCCTGTCGAAGCGTGTGTCGGCCACAT83160.12078148935079369No Hit
ATCACTGCGACGTCGATGACCTGGCCCATGTCGAAGCGTGTGTCGGCCAC80250.11655500866283301No Hit
CACTGCGACGTCGATGACCTGGACCCTGTCGAAGCGTGTGTCGGCCACAT71940.10448557412092468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGA100.0072532087143.912341
GAGTGTA100.0072532087143.912341
GAGTGCA202.2128988E-6143.912341
AGATCCG100.0072532087143.912341
GAGTCGC351.2732926E-11143.912341
GAGTAAC100.0072532087143.912341
AGGACGC100.0072532087143.912342
GATTTAG100.0072532087143.912342
GATCACG100.0072532087143.912341
AGTGCAA202.2128988E-6143.912342
AGTGAAC100.0072532087143.912342
GAGCGCG100.0072532087143.912341
AGCGCGA100.0072532087143.912342
AGATTTA100.0072532087143.912341
GTGTAGA100.0072532087143.912343
GCGTCGT100.0072532087143.912343
GTGAACG100.0072532087143.912343
AGTGTAG100.0072532087143.912342
CGGACGC100.0072532087143.912344
GAGTGCG246500.0143.912321
Produced by FastQC (version 0.11.5)