FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005099986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005099986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349314
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGCA5828129.17913336779375No Hit
TGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCG3750005.906149861229102No Hit
CTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC3156534.971450459057467No Hit
ATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC2958474.65951124798679No Hit
GTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC2941974.633524188597383No Hit
GTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2750704.332279046208772No Hit
GAGTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG2674084.2116045922441385No Hit
ATCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCC2593614.084866491088643No Hit
TCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCG2560134.032136385127591No Hit
CTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2510963.954694948147154No Hit
TCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACA2338153.682523812808754No Hit
ATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2277863.5875686727731533No Hit
ATCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCAC2225293.504772326585203No Hit
CACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG2194293.455948154399042No Hit
GAGTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT2002103.15325403657781No Hit
GACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGG1874442.952192945568608No Hit
CACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT1734972.732531419929775No Hit
GCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGCAG1727442.720671871008427No Hit
ACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1695372.6701624773951957No Hit
GATCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACC1586822.4991991260788176No Hit
AGTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1449812.283412034748951No Hit
ACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1291992.0348497491225035No Hit
GATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1215301.9140650470271274No Hit
GATCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCA1196451.8843768003913495No Hit
AGATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG1072601.6893163576411563No Hit
GATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1049061.6522414862456007No Hit
AGTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1041951.6410434261087106No Hit
AGATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT716241.1280588737617954No Hit
GAGTGCGACGTCGATGACCTGGACCCTGTCGAAGCGTGTGTCGGCCACAT68140.10731868041177361No Hit
ATCACTGCGACGTCGATGACCTGGCCCATGTCGAAGCGTGTGTCGGCCAC63850.10056204497052752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGC202.2126187E-6143.915821
AGATCGT202.2126187E-6143.915821
AGATACG100.0072526727143.915821
GTGGGAC100.0072526727143.915822
CTGAGAC100.0072526727143.915824
GGTCACT100.0072526727143.915821
AGTGGGA100.0072526727143.915821
CTGCGAA100.0072526727143.915821
GAACACT100.0072526727143.915821
GAGTGCG210650.0143.91581
AGATCTA450.0143.91581
AGATCGA900.0143.91581
GGATGCG151.2676505E-4143.91581
GGATGAC151.2676505E-4143.91581
GAGTCGC650.0143.91581
GAGTACG750.0143.91581
AGACGTG351.2732926E-11143.91583
GTACGAC151.2676505E-4143.91581
AGATGCG77700.0143.91581
GAGTGAC273600.0143.88951
Produced by FastQC (version 0.11.5)