FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005099989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005099989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7582691
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGCA6017557.935902966374339No Hit
TGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCG4087895.3910808181422665No Hit
CTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC3733814.924122583921724No Hit
GTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC3577634.718153489308743No Hit
GAGTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG3323174.382573416218595No Hit
ATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGC3322524.381716200752477No Hit
ATCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCC3270484.31308621174198No Hit
TCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCG3130194.128072738293041No Hit
TCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACA3085194.068727052177123No Hit
GTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC3027703.9929096411814755No Hit
ATCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCAC3021423.9846276209857425No Hit
CTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2784043.6715725327591486No Hit
CACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG2705253.5676648303352994No Hit
GAGTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT2665863.5157175730884984No Hit
ATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCC2441113.2193188407651054No Hit
CACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT2315323.0534278661757415No Hit
GCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATGCAG2126882.804914508582771No Hit
GACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGG2097672.7663925643284157No Hit
ACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC2042512.693647941080548No Hit
GATCACTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACC2000082.6376915530383607No Hit
AGTGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1844172.432078532542075No Hit
ACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1749132.306740443465255No Hit
GATCACTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCA1623832.1414956774580425No Hit
AGTGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1444851.9054581018796626No Hit
GATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGC1372401.8099115472330336No Hit
AGATGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGG1337561.763964798249065No Hit
GATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACATG1295821.7089183773939884No Hit
AGATGCGACGTCGATGACCTGGACCATGTCGAAGCGTGTGTCGGCCACAT989101.304418180827888No Hit
ATCACTGCGACGTCGATGACCTGGCCCATGTCGAAGCGTGTGTCGGCCAC100020.1319056783403148No Hit
GAGTGCGACGTCGATGACCTGGACCCTGTCGAAGCGTGTGTCGGCCACAT92270.12168503239812885No Hit
CACTGCGACGTCGATGACCTGGACCCTGTCGAAGCGTGTGTCGGCCACAT78830.10396045414484119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA151.267713E-4143.91431
AGATCGC202.2127624E-6143.91431
ACTGCGG100.0072529265143.91432
TCCGCGC100.0072529265143.91434
AGATACG400.0143.91431
GAGTCGT100.0072529265143.91431
GAGTCGC650.0143.91431
GAGTACG450.0143.91431
GAGAGCG100.0072529265143.91431
TGCGGAC100.0072529265143.91434
CTGCGGA100.0072529265143.91433
AGTGCCA100.0072529265143.91432
CACTCGG100.0072529265143.91433
TGACCGC151.267713E-4143.91432
GAGGTGC202.2127624E-6143.91431
AGTGACT100.0072529265143.91431
GAGCGCG100.0072529265143.91431
TCAGACG202.2127624E-6143.91431
AGCGCGA100.0072529265143.91432
AGTCTCA151.267713E-4143.91432
Produced by FastQC (version 0.11.5)