FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63705874
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTCGTATG5323480.8356340892521151TruSeq Adapter, Index 15 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1016350.090.448131
TCGGAAG1035200.088.715822
CGGAAGA1064750.086.150183
AGAGCAC1395750.065.730187
AGCACAC1441950.063.473369
GAGCACA1463700.062.678768
GAAGAGC1496350.061.601815
AAGAGCA1826450.050.583436
GGAAGAG2019500.045.8198054
TATGCCG742800.026.08394445-49
CTCGTAT760700.025.42633240-44
TCTCGTA703550.024.99398640-44
CGTATGC761050.024.9288245-49
ATGCCGT780050.024.63946345-49
GCCGTCT780050.023.92389550-54
TCGTATG778500.023.8802340-44
TGCCGTC779300.023.65663145-49
ATCTCGT741750.023.60711140-44
CGTCTTC819000.023.21100450-54
CCGTCTT818650.023.10580650-54
Produced by FastQC (version 0.11.5)