FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19214686
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG112100.014.5498889
GAGCGTC134850.012.148998
CGCCGTA29550.011.28868655-59
AGAGCGT149900.011.0747397
ATCGGAA165150.010.1231831
TCGGAAG169400.09.9279752
AAGAGCG173850.09.8400786
TCGCCGT35350.09.64165355-59
CGGAAGA177400.09.4803143
GCCGTAT37250.09.22773555-59
GTCGCCG40900.08.86522455-59
TGGTCGC44100.08.31850450-54
GTGGTCG50100.07.32227650-54
CGTATCA48350.07.288975760-64
CCGTATC47050.07.120743355-59
GGTCGCC50850.06.90061450-54
AGATCGG164500.06.344805145
CGTCGTG100800.06.2557645145
GATCGGA151650.06.0221252145
GAAGAGC304850.05.944625