FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18723482
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTATG512620.2737845449900825TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA198800.042.4238851
TCGGAAG204300.041.3131942
CGGAAGA212450.039.583463
AGAGCAC308350.027.7653687
AGCACAC316950.026.8290869
GAGCACA332100.025.7797378
GAAGAGC332500.025.6615165
TATGCCG93050.021.09953545-49
AAGAGCA420800.020.569616
CTCGTAT100000.019.67496940-44
CGTATGC99850.019.38669645-49
ATGCCGT103750.018.9793945-49
TCTCGTA98150.018.74586540-44
GCCGTCT108650.017.76282350-54
GGAAGAG490550.017.6005384
ATCTCGT107750.017.1429840-44
TCGTATG107350.016.40999440-44
CGTCTTC119950.016.40375350-54
TGCCGTC106800.016.40084645-49
CCGTCTT121300.016.11360550-54
Produced by FastQC (version 0.11.5)