FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16979129
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTATG515680.30371404799386353TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA201750.043.923341
TCGGAAG205550.043.280842
CGGAAGA208900.042.5855183
AGAGCAC291250.030.5906037
AGCACAC298500.029.9204759
GAGCACA307800.028.9928918
GAAGAGC325700.027.4445
AAGAGCA402850.022.4581176
TATGCCG95650.021.51052545-49
CTCGTAT100850.020.35811840-44
CGTATGC101250.019.97711645-49
TCTCGTA98850.019.59657140-44
ATGCCGT104700.019.52657345-49
GGAAGAG470650.019.4716854
GCCGTCT107900.018.39689450-54
CGTCTTC116750.017.56123550-54
ATCTCGT109900.017.54705440-44
TCGTATG106750.017.27704640-44
CCGTCTT117800.017.19545450-54
TGCCGTC107400.016.80810245-49
Produced by FastQC (version 0.11.5)