FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75188761
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA474100.023.30061
TCGGAAG489350.022.509692
CGGAAGA522150.021.3570633
TATGCCG169650.014.21313145-49
AGAGCAC920800.012.5257217
CTCGTAT194550.012.5049940-44
CGTATGC194000.012.32452945-49
AGCACAC966500.011.8732919
TCTCGTA193500.011.74864240-44
GAGCACA999050.011.689628
ATGCCGT216700.011.2609945-49
GAAGAGC1045400.011.2408295
TCGTATG215450.010.84778740-44
TGCCGTC225850.010.32327145-49
GCCGTCT232700.010.2561350-54
ATCTCGT238850.09.5179540-44
CGTCTTC274350.09.05849450-54
CCGTCTT280050.08.68255650-54
AAGAGCA1426450.08.34985356
GGAAGAG1661850.07.40740784
Produced by FastQC (version 0.11.5)