FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74343042
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGCACGAGTGTAGATCTC1751590.2356091374361571Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGCACGAGTGTAGATCT1408100.1894057550133609Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG537650.050.1201139
GATCGGA457450.046.8133741
GAGCGTC607150.044.4785468
AGAGCGT656750.041.2518427
ATCGGAA671950.040.2865561
TCGGAAG697250.038.862462
CGGAAGA739950.036.673823
AAGAGCG752800.036.2196356
CGCCGTA393700.024.3092555-59
TCGCCGT408800.023.33328855-59
GTCGCCG434000.022.0786955-59
GCCGTAT435150.022.03367255-59
GAAGAGC1280950.021.6933675
TGGTCGC456950.021.20032550-54
CGTATCA468950.020.41919760-64
GTGGTCG477050.020.38305950-54
CGAGTGT509900.019.17481635-39
TGCACGA509450.019.13663930-34
ACGAGTG519150.018.83875335-39
GCGTCGT518450.018.56104910-14
Produced by FastQC (version 0.11.5)