FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97204060
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTTCAGGTGTAGATCTC3945420.4058904535468992Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1278500.048.109589
GAGCGTC1396700.044.1014258
AGAGCGT1488200.041.5264637
AAGAGCG1654500.037.5452966
ATCGGAA1649900.037.4906231
TCGGAAG1665100.037.2124822
CGGAAGA1715900.036.1199153
GAAGAGC2287950.027.358295
CGCCGTA617600.021.42096755-59
GGAAGAG3037350.020.901814
TCGCCGT649900.020.238155-59
GCCGTAT663200.020.00277355-59
GTCGCCG712650.018.71043255-59
TGGTCGC725000.018.67945750-54
CGTATCA710650.018.47745760-64
GTGGTCG762900.017.79329550-54
CCGTATC700550.017.4274655-59
GGTCGCC753550.016.54400650-54
TCTCGGT851100.016.17771745-49
TACGTTC876500.015.39933830-34
Produced by FastQC (version 0.11.5)