FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59306273
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTAATCATCTCGTATG686940.11582923108319418TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA301550.037.487741
TCGGAAG319750.035.2463232
CGGAAGA340300.033.0539363
AGAGCAC690550.016.7342457
TATGCCG147800.016.67762845-49
AGCACAC728850.015.9046189
GAGCACA753550.015.4217778
GAAGAGC772800.015.1782915
CTCGTAT165000.014.89493440-44
ACCGGTA163900.014.54364930-34
TCTCGTA158100.014.50866340-44
CCGGTAA171550.014.32614930-34
CGTATGC165950.014.31187145-49
CGGTAAT176000.013.95568730-34
TCGTATG177350.013.481626540-44
ATGCCGT186500.013.15471145-49
CACCGGT181900.013.008821530-34
TGCCGTC185400.012.79479545-49
GCCGTCT188200.012.36682250-54
ATCTCGT196700.011.55094340-44
Produced by FastQC (version 0.11.5)