FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16683374
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC9803.2610114E-710.3551453
CTCGGTG71350.07.6191619
GTAGATC108150.07.5066423
ATCGGTG18556.772043E-77.0334519
TCTCGGT63800.06.70303068
GATGTCG19306.393778E-56.0093296
GCGTCGT115400.05.905646145
GTCGTGT116650.05.8423615145
GAGCGTC145500.05.8299956145
GCGTAGA17657.827486E-45.76518441
ATCTCCG31552.5193003E-85.74379257
TCGTGTA113100.05.7052245145
CGCCGTA27050.05.628381755-59
GTGTAGA147400.05.52268741
AGCGTCG123900.05.44065959
CGTCGTG112250.05.4254813145
GATCGGA177150.05.3204403145
TAGATCT146850.05.23222164
ATCGGAA182300.05.209907145
ATGTCGG20950.00123959195.1899767
Produced by FastQC (version 0.11.5)