FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16557232
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCAGACGTGTAGATCTC370790.22394443708948453Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCAGACGTGTAGATCT199150.12027976656967783Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG148900.037.1957559
GAGCGTC169450.032.899058
AGAGCGT180700.031.0514187
ATCGGAA192500.029.1557791
TCGGAAG196800.028.5908532
AAGAGCG199750.028.2716166
CGGAAGA204600.027.5312443
CGCCGTA73800.022.76967255-59
TCGCCGT77700.021.51483355-59
GCCGTAT82050.020.62158255-59
GTCGCCG84000.019.95300355-59
GATCGGA150200.019.2923551
TGGTCGC88500.019.15177750-54
CGTATCA89850.018.70221160-64
GTGGTCG91150.018.49953850-54
GAAGAGC316300.018.1291435
TCTCGGT105200.016.40125845-49
GACGTGT110350.015.6082135-39
CTCGGTG114600.015.13186845-49
CGGTGGT114750.014.9349150-54
Produced by FastQC (version 0.11.5)