FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187314435
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCAGACGTGTAGATCTC3563830.19025922908717632Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCAGACGTGTAGATCT1910080.1019718528366487Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1560100.035.8498089
GAGCGTC1768900.031.6612138
AGAGCGT1938200.029.0188127
ATCGGAA2079300.027.1172471
TCGGAAG2115200.026.7593292
CGGAAGA2209450.025.6002043
AAGAGCG2216000.025.5177526
CGCCGTA772250.022.03491655-59
TCGCCGT827850.020.53750655-59
GCCGTAT863250.019.80945255-59
GTCGCCG885500.019.28224255-59
TGGTCGC942950.018.18909350-54
GATCGGA1643150.018.0339641
CGTATCA962800.017.79731860-64
GTGGTCG978900.017.61139950-54
GAAGAGC3424000.016.8811135
TCTCGGT1126500.015.57915145-49
GACGTGT1174300.014.87233835-39
CTCGGTG1222300.014.32491345-49
CGGTGGT1233650.014.12615650-54
Produced by FastQC (version 0.11.5)