FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73108425
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTCCAAGTGTAGATCT2980940.40774233612610306Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTCCAAGTGTAGATCTC1534440.20988552276977654Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA620450.073.712311
GAGCGTC755000.060.211849
AGAGCGT796200.057.2600148
ATCGGAA810300.056.467632
TCGGAAG832450.054.9785083
CGGAAGA881200.051.934154
AAGAGCG892300.051.2718547
AGCGTCG685000.033.716879
GAAGAGC1388850.033.2122736
CGCCGTA564850.024.220655-59
TCGCCGT577200.023.62700855-59
GGAAGAG1993000.023.511815
GCCGTAT600250.022.7970155-59
TGGTCGC638950.021.56372650-54
CGTATCA634750.021.53994460-64
GTCGCCG639850.021.41557155-59
GTGGTCG661600.020.85835850-54
GCGTCGT663750.020.61472310-14
CGTCGTG666200.020.5563210-14
TCTCGGT700000.019.91770245-49
Produced by FastQC (version 0.11.5)