FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116072715
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG423600.012.8017099
GAGCGTC536550.010.2010888
AGAGCGT598250.09.3186517
CGCCGTA138350.09.21049555-59
ATCGGAA642650.08.8375361
TCGGAAG682050.08.2809982
AAGAGCG725800.07.9705216
CGGAAGA728800.07.83933543
TCGCCGT171950.07.43600555-59
GTCGCCG200700.06.789749655-59
GCCGTAT201600.06.75224755-59
ACCGCGC377200.06.2898141
CGCGATC213000.06.02497243
TGGTCGC234650.05.955639450-54
GCGCGAT209250.05.7171542
CGTATCA250950.05.58611660-64
GTGGTCG257950.05.496361350-54
TCCGGTA246450.05.359860430-34
CGGTAGT251700.05.328074535-39
CTCACGC1141100.05.28005843
Produced by FastQC (version 0.11.5)