FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59778018
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTCGTATG975570.16319878655060127TruSeq Adapter, Index 23 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA406150.040.370131
TCGGAAG421500.038.8956832
CGGAAGA446950.036.7261123
AGAGCAC771750.021.7015517
AGCACAC819600.020.566369
GAGCACA841550.020.021548
GAAGAGC853550.019.6897095
TATGCCG200350.019.1412645-49
CTCGTAT217300.017.59480940-44
CGTATGC216700.017.50978345-49
TCTCGTA211550.016.85355640-44
ATGCCGT234800.016.3513645-49
GCCGTCT243200.015.47071650-54
AAGAGCA1152500.014.8087196
ATCTCGT243100.014.74377340-44
TCGTATG233650.014.55230240-44
TGCCGTC238750.014.14433745-49
CGTCTTC279400.013.621950-54
CCGTCTT285050.013.39257350-54
GGAAGAG1342900.012.7733464
Produced by FastQC (version 0.11.5)