FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20476700
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACTCTATCTCGTATG1068060.5215977183823565TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA237100.074.773341
TCGGAAG244200.072.449792
CGGAAGA247700.071.208123
AGAGCAC356450.049.9087457
AGCACAC370900.047.7299049
GAGCACA377100.047.002728
GAAGAGC392600.045.16555
AAGAGCA493900.036.2395486
GGAAGAG561000.032.008394
TATGCCG150800.023.73086445-49
CTCGTAT157850.022.5867340-44
CGTATGC158450.022.328945-49
ATGCCGT161300.022.1231545-49
TCTCGTA153050.022.00671840-44
TCGTATG162900.021.85982540-44
ACGCTAC164350.021.569930-34
CGCTACT164050.021.5386430-34
TGCCGTC165250.021.38374545-49
GCCGTCT165950.021.09213850-54
TATCTCG157100.021.03330640-44
Produced by FastQC (version 0.11.5)