FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41286554
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTAGCGTGTAGATCTC1832570.44386605866888285Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG340200.081.569649
GAGCGTC380350.073.016278
AGAGCGT408800.068.1121147
ATCGGAA426600.065.7004551
TCGGAAG434950.064.336952
CGGAAGA461250.060.703543
AAGAGCG468750.059.4853826
GAAGAGC737550.038.2191545
GGAAGAG1076200.026.5099124
CGCCGTA223950.024.46703555-59
TCGCCGT234350.023.38742455-59
GCCGTAT244850.022.50292855-59
GTCGCCG247750.022.33901655-59
TGGTCGC261600.021.51901850-54
CCGTATC256150.021.30077655-59
GGTCGCC266100.020.89346550-54
GTGGTCG272000.020.82421950-54
CGTATCA264700.020.68945360-64
GTAGCGT270850.020.46313735-39
TAGCGTG287100.019.54729735-39
Produced by FastQC (version 0.11.5)