FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37433167
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTGAGGTGTGTAGATCTC400190.10690786595748097Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG203650.031.14699
GAGCGTC237050.026.880568
AGAGCGT266400.024.0006947
ATCGGAA271400.023.560561
TCGGAAG283300.022.6188322
CGGAAGA304500.021.0915833
AAGAGCG317750.020.3957216
CGCCGTA96750.014.02815655-59
TCGCCGT106050.012.81163855-59
GCCGTAT114850.011.95624255-59
GAAGAGC567600.011.7115425
GTCGCCG120500.011.34750655-59
TGGTCGC131500.010.68365450-54
CGTATCA130050.010.58078860-64
CCGTATC124000.010.40744355-59
GTGGTCG141700.09.89414850-54
GGTCGCC138850.09.52293250-54
TCTCGGT168600.08.62456245-49
GGAAGAG856250.08.0254714
TCGGTGG174750.07.71541845-49
Produced by FastQC (version 0.11.5)