FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005100951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46847048
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTATG1323790.28257703665767797TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA495050.042.6969031
TCGGAAG502250.042.2245562
CGGAAGA520150.040.799143
AGAGCAC755350.028.3226767
AGCACAC784250.027.297789
GAGCACA803250.026.6607918
GAAGAGC837000.025.5594225
TATGCCG234200.020.60389745-49
AAGAGCA1072900.020.2101946
CTCGTAT248100.019.5081840-44
CGACGTC245800.019.4839830-34
ACGACGT247200.019.22699730-34
CGTATGC247200.019.20948645-49
TCTCGTA246650.019.10554540-44
ATGCCGT258300.018.53555745-49
CACGACG258300.018.2379630-34
TATCTCG257950.017.88635640-44
GCCGTCT263400.017.78627450-54
GGAAGAG1220900.017.7616774
CGTCATA268600.017.64077635-39
Produced by FastQC (version 0.11.5)