FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005100993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005100993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65427445
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTGACCTGTGTAGATCTC6074000.9283565940867782Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1198250.078.3832249
GAGCGTC1271650.073.8291248
AGAGCGT1324950.071.034227
ATCGGAA1419900.066.879071
TCGGAAG1440650.065.878482
AAGAGCG1448750.065.369446
CGGAAGA1471500.064.4485553
GAAGAGC1851550.051.2780765
GGAAGAG2381000.040.094624
CGCCGTA820800.023.48908655-59
GCCGTAT847750.022.74407855-59
TCGCCGT839150.022.6575455-59
CGTATCA868750.021.71193360-64
TGGTCGC933500.021.29366150-54
CCGTATC864600.021.21085255-59
GTCGATC48350.020.8409563
GTGGTCG966500.020.5381150-54
GTCGCCG940600.020.5004755-59
TCTCGGT1004750.020.19957445-49
GGTCGCC984050.019.28191450-54
Produced by FastQC (version 0.11.5)