FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87954547
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA596750.09.5566011
TCGGAAG617100.09.1644392
CGGAAGA650500.08.8824393
TATGCCG164850.08.51409345-49
CGTATGC191300.07.38237345-49
CTCGTAT196150.07.325559640-44
TCTCGTA196550.06.80901240-44
ATGCCGT214850.06.62042245-49
TATCTCG212950.06.386759840-44
GCCGTCT231200.06.277836350-54
TGCCGTC220400.05.6905845-49
AGAGCAC1110700.05.62621837
AGCACAC1155500.05.57123339
TCGTATG226500.05.53736740-44
GATCGGA541150.05.521456145
ATCTCGT248750.05.51421240-44
CGTCTTC280250.05.339465650-54
GAGCACA1194850.05.31494868
CCGTCTT287850.05.208564350-54
GAAGAGC1247500.05.02666475
Produced by FastQC (version 0.11.5)