FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85149374
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA436550.028.87911
TCGGAAG465250.027.1251432
CGGAAGA508750.024.8713863
TATGCCG198600.014.31828645-49
AGAGCAC986100.013.3357327
AGCACAC1041150.012.6584669
CTCGTAT224550.012.566540-44
GAGCACA1062000.012.4167728
CGTATGC226450.012.37164645-49
GAAGAGC1093300.012.0811865
TCTCGTA219000.011.96461140-44
ATGCCGT245200.011.55571445-49
GCCGTCT265750.010.40999350-54
TCGTATG251550.010.35877240-44
TGCCGTC260450.09.99941745-49
ATCTCGT271400.09.9003440-44
ACCGTCT312700.09.22290130-34
GTCACCG315450.09.11447225-29
AAGAGCA1539600.08.8992716
CACCGTC337650.08.42115230-34
Produced by FastQC (version 0.11.5)