FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79890141
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTATG2441110.30555835419041255TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA871650.043.584271
TCGGAAG883700.043.0222632
CGGAAGA914550.041.5509573
AGAGCAC1332050.028.8922397
AGCACAC1360200.028.2776579
GAGCACA1398250.027.5605288
GAAGAGC1447500.026.633075
TATGCCG415950.021.085245-49
AAGAGCA1855150.021.0696936
CTCGTAT444700.019.79052540-44
CGACGTC441900.019.6374530-34
TCTCGTA441100.019.49186540-44
CGTATGC446700.019.3383745-49
ACGACGT446050.019.30521830-34
ATGCCGT462450.018.88353745-49
GGAAGAG2092050.018.6770464
TATCTCG455100.018.43985240-44
CACGACG466900.018.26611130-34
GCCGTCT470500.018.03979750-54
CGTCATA473800.017.97983735-39
Produced by FastQC (version 0.11.5)