FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73831043
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG282750.025.6312799
GAGCGTC354000.020.6564548
AGAGCGT386500.018.9569197
ATCGGAA419650.017.5442391
TCGGAAG440800.016.7695062
AAGAGCG458250.016.1466466
CGGAAGA475300.015.6743433
CGCCGTA105900.014.98461555-59
TCGCCGT127050.012.490128555-59
GTCGCCG137900.011.62300155-59
GCCGTAT146650.011.03820855-59
TGGTCGC160900.010.14193850-54
GTGGTCG176200.09.43400650-54
CGTATCA183350.09.05793260-64
GGTCGCC171150.08.98415250-54
CCGTATC170600.08.82600155-59
GTCGTTG198750.08.23233530-34
TCGTTGC207450.08.11762330-34
GAAGAGC989150.08.0299315
GTGTCGT211550.07.89167625-29
Produced by FastQC (version 0.11.5)