FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88525850
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA474700.025.2491321
TCGGAAG498800.024.0686532
CGGAAGA537400.022.3935743
TATGCCG194200.013.49260345-49
AGAGCAC1033900.012.0254697
CTCGTAT220400.011.94077440-44
CGTATGC221500.011.84272645-49
AGCACAC1086200.011.5398949
TCTCGTA218350.011.28255840-44
GAGCACA1120400.011.2588188
GAAGAGC1154350.010.9404185
ATGCCGT245100.010.83257945-49
TATCTCG237150.010.2903140-44
GCCGTCT255050.010.28546850-54
TCGTATG246100.010.09283540-44
TGCCGTC250300.09.89495645-49
ATCTCGT263250.09.58403340-44
CGTCTTC308600.08.70743750-54
CCGTCTT308900.08.572227550-54
AAGAGCA1635050.08.0475196
Produced by FastQC (version 0.11.5)