FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69320161
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG321650.030.2912949
GAGCGTC389750.025.2041238
AGAGCGT435300.022.6167147
ATCGGAA474550.020.8528751
TCGGAAG493100.019.9678552
AAGAGCG510200.019.3680426
CGGAAGA524850.018.9663413
CGCCGTA121150.016.68164855-59
TCGCCGT136050.014.84404355-59
GTCGCCG150600.013.70833255-59
GCCGTAT162050.012.677114555-59
CGTTCGT161450.012.6525535-39
TGGTCGC172950.012.20577550-54
GTGGTCG184150.011.42405350-54
GGTCGCC179500.011.31613850-54
CCGTATC183600.010.99173355-59
CGTATCA194500.010.64393460-64
CCGTTCG188600.010.5935630-34
GAAGAGC989950.010.5752255
GTAACCG210900.09.89966130-34
Produced by FastQC (version 0.11.5)