FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18565646
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACTCTATCTCGTATG1067450.5749597940195563TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA236100.072.170151
TCGGAAG240500.070.905072
CGGAAGA246450.069.191733
AGAGCAC339200.050.330957
AGCACAC357000.047.7402279
GAGCACA363100.047.0180638
GAAGAGC371350.045.9344525
AAGAGCA459200.037.3519176
GGAAGAG521450.033.1327174
TATGCCG144550.023.87345945-49
CTCGTAT150800.022.88389840-44
TCTCGTA144850.022.71278440-44
CGTATGC150800.022.67247645-49
ATGCCGT155700.021.99620645-49
TCGTATG155850.021.84467940-44
TATCTCG149000.021.72012540-44
CGCTACT158800.021.40201830-34
ACGCTAC159050.021.36837630-34
TGCCGTC158250.021.31191645-49
GCCGTCT158600.021.26536650-54
Produced by FastQC (version 0.11.5)