FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65547656
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCGAGTGTGTAGATCTC659200.10056805082396844Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG364650.028.2503079
GAGCGTC434000.023.8697648
AGAGCGT476550.021.9970367
ATCGGAA504200.020.5250821
TCGGAAG526200.019.7695942
AAGAGCG562000.018.742926
CGGAAGA568100.018.3370863
CGCCGTA165900.014.8225555-59
TCGCCGT187850.013.10599355-59
GCCGTAT198250.012.66712755-59
GTCGCCG220400.011.3151555-59
CGTATCA227050.011.02931460-64
TGGTCGC230050.010.99148950-54
GAAGAGC996550.010.9774315
GTGGTCG252100.010.10488150-54
CCGTATC218000.09.65060155-59
TGATCGA265000.09.60821130-34
CGAGTGT277050.09.16410635-39
GTGATCG284600.08.91593730-34
TCTCGGT298800.08.76825345-49
Produced by FastQC (version 0.11.5)