FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69401599
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTGATCTCGTAT3022170.43546114838074557TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTGATCTCGTATG1540740.22200353049502503TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA605500.081.27191
ATCGGAA798050.061.6530272
TCGGAAG818550.060.044963
CGGAAGA850000.057.831824
AGAGCAC1232750.039.9516568
GAGCACA1319300.037.3523649
GAAGAGC1342200.036.8181156
AAGAGCA1700100.029.2122927
GGAAGAG1886050.026.570855
TATGCCG599900.024.98814245-49
CTCGTAT616500.024.2189540-44
CGTATGC620000.024.04474645-49
TCTCGTA593250.023.9754540-44
ATGCCGT642500.023.24558645-49
GCCGTCT646550.022.82803750-54
ATCTCGT635700.022.32655140-44
CGTCTTC685750.021.6901650-54
CCGTCTT693300.021.3807550-54
CGTCTGA742800.020.03341715-19
AGCACAC1287300.019.9738589
Produced by FastQC (version 0.11.5)