FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64446172
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACGAAATCTCGTATG664250.10307051286149314TruSeq Adapter, Index 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA458250.024.7624721
TCGGAAG477250.023.7963582
CGGAAGA495900.022.9146963
AGAGCAC829300.014.1549797
TATGCCG172300.013.84539645-49
AGCACAC877000.013.3189819
GAGCACA893900.013.0914928
CTCGTAT189450.012.66950440-44
GAAGAGC937550.012.6137745
CGTATGC189800.012.44664245-49
TCTCGTA185450.011.8642140-44
TCGTATG202100.011.5752740-44
ATGCCGT209000.011.42110545-49
TGCCGTC218850.010.7282645-49
GCCGTCT221450.010.51729550-54
ATCTCGT227200.09.82440740-44
AAGAGCA1260550.09.64010056
CCCAACG255250.09.41391130-34
CGTCTTC255850.09.39210650-54
CCGTCTT262600.09.06238150-54
Produced by FastQC (version 0.11.5)