FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90738866
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG366950.021.1383449
TCTCGGT305900.020.4754248
GCGTAGA113550.020.2584231
GTAGATC536650.015.3330313
CGTAGAT119200.013.5069342
CTCGGCG123100.013.0734149
GATCTCG605400.012.2380896
ATATCGG90800.011.65644557
AACTCGG186950.011.4779777
GATATCG97750.011.4211296
GTGTAGA738000.011.3336391
ATCGCGG52100.011.2705837
TATCGGT82750.011.2134868
GAACTCG187800.010.4611356
ATCTCGG733350.010.43882757
GATGTCG112350.010.259576
GATCCCG152200.010.2406836
TCTCGGG223750.010.173388
ACTCGGT159450.010.1386228
CTCGGGG313300.010.0420749
Produced by FastQC (version 0.11.5)