FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16630935
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGG15152.3101165E-109.570157
TATCGGT12156.294904E-78.94986258
GATATCG17009.94075E-66.8229546
TCTCGGT61800.06.45172178
GTAGATC106100.06.35433773
GTCGGTG23206.2041E-76.24947269
GATCCCG27104.090907E-86.1526186
ATCGGTG18904.7099362E-56.13704829
GCGTAGA16954.4315887E-46.027291
GTCGTGT109050.05.916903145
GAGCGTC134350.05.881918145
CTCGGTG70500.05.6555529
GTGTAGA141800.05.6093691
CGTCGTG106350.05.589931145
CGTAGAT19856.001684E-45.47908262
GATGTCG18750.0017406955.41287666
TGTCGGT20257.863299E-45.36991748
AGCGTCG114100.05.337326145
GCGTCGT107050.05.322893140-144
TAGATCG63150.05.280981145
Produced by FastQC (version 0.11.5)