FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81240053
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA575050.010.8930521
TCGGAAG593300.010.5343872
CGGAAGA618150.010.1096153
TATGCCG152250.08.75200145-49
CTCGTAT170300.07.833015440-44
CGTATGC173150.07.695593445-49
TCTCGTA175300.07.270474440-44
TCGTATG191800.06.803771540-44
ATGCCGT200750.06.70257345-49
AGAGCAC1040950.06.4210657
TGCCGTC209150.06.357122445-49
AGCACAC1074150.06.29012739
GCCGTCT217300.06.218959350-54
GAGCACA1109150.06.2092878
GAAGAGC1168100.05.8896515
ATCTCGT221900.05.84819140-44
GATCGGA516550.05.812625145
AGATCGG560300.05.6176343145
CGTCTTC259300.05.43532250-54
CCGTCTT269600.05.233044650-54
Produced by FastQC (version 0.11.5)