FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68369507
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA323100.018.306681
ATCGGAA371650.016.1329172
TCGGAAG383800.015.5829353
CGGAAGA413250.014.595164
TATGCCG143750.013.09189945-49
CTCGTAT163900.011.47375340-44
CGTATGC164850.011.39860945-49
TCTCGTA165000.010.67669340-44
ATGCCGT189200.09.954645-49
GCCGTCT198000.09.49016250-54
ATCTCGT198750.08.87826140-44
CGTGGTA226850.08.39248330-34
CGTCTTC239900.08.14692150-54
CCGTCTT237900.08.09961550-54
AGAGCAC791500.08.0324148
GTCACGT256600.07.526439725-29
GAGCACA868800.07.4512499
AGTCACG261000.07.382891725-29
GAAGAGC893050.07.24080476
TCGTATG188250.06.68529545-49
Produced by FastQC (version 0.11.5)