FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93554609
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA425150.018.2993621
ATCGGAA486700.016.1017362
TCGGAAG506600.015.5552533
CGGAAGA542850.014.56992154
TATGCCG189850.012.88541845-49
CTCGTAT218400.011.14258840-44
CGTATGC221000.011.01016145-49
TCTCGTA216700.010.46036440-44
ATGCCGT251500.09.88250145-49
GCCGTCT259550.09.40746250-54
CGTGGTA295700.08.44358330-34
ATCTCGT271300.08.3819140-44
AGAGCAC1062200.07.88283828
CGTCTTC321350.07.86900150-54
GTCACGT326050.07.67962425-29
CCGTCTT328200.07.647330850-54
AGTCACG333150.07.515958325-29
GAGCACA1171100.07.26126249
GAAGAGC1210250.07.12223776
TCGTATG242550.06.791584545-49
Produced by FastQC (version 0.11.5)