FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005101176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61002690
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA321800.021.2013821
ATCGGAA363150.018.8993682
TCGGAAG379750.018.1867623
CGGAAGA405800.017.0192764
TATGCCG146150.014.51801545-49
CGTATGC163200.012.9746345-49
CTCGTAT166650.012.71564240-44
TCTCGTA168050.011.77308740-44
ATGCCGT182300.011.72655145-49
GCCGTCT189650.011.24920350-54
ATCTCGT198050.010.04096640-44
CGTGGTA219600.09.80710930-34
AGAGCAC738500.09.7531978
CGTCTTC229050.09.50400150-54
CCGTCTT229550.09.4201650-54
GAGCACA795150.09.2679369
GTCACGT243150.08.91708725-29
GAAGAGC832300.08.7933366
AGTCACG248950.08.73262425-29
TCGTATG182000.07.899602445-49
Produced by FastQC (version 0.11.5)