FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005101178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005101178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80728093
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGACGTTATCTCGTATG2396050.2968049796494016TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1002900.040.6650541
TCGGAAG1016850.040.111322
CGGAAGA1052000.038.8308143
AGAGCAC1437850.028.6683087
AGCACAC1484700.027.7148469
GAGCACA1511250.027.2567258
GAAGAGC1577800.026.111665
TATGCCG460000.021.64428745-49
AAGAGCA1972400.021.1303226
CTCGTAT484900.020.46136540-44
CGTATGC484250.020.13823345-49
TCTCGTA468400.019.83562340-44
ATGCCGT505050.019.48397645-49
CGTTATC479250.019.03908235-39
TATCTCG484650.018.76368140-44
GCCGTCT513250.018.69535650-54
GACGTTA515350.018.55789835-39
GGAAGAG2255200.018.5444414
ATCTCGT514000.018.0166540-44
CGTCTTC560800.017.5522950-54
Produced by FastQC (version 0.11.5)